[Emergent viruses: SARS-associate coronavirus and H5N1 influenza virus].
Identifieur interne : 000414 ( France/Analysis ); précédent : 000413; suivant : 000415[Emergent viruses: SARS-associate coronavirus and H5N1 influenza virus].
Auteurs : A. Goffard [France] ; M. Lazrek ; C. Schanen ; P-E Lobert ; L. Bocket ; A. Dewilde ; D. HoberSource :
- Annales de biologie clinique [ 0003-3898 ]
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Grippe humaine, Syndrome respiratoire aigu sévère.
- pathogénicité : Sous-type H5N1 du virus de la grippe A, Virus du SRAS.
- épidémiologie : Grippe humaine, Syndrome respiratoire aigu sévère.
- Humains, Maladies transmissibles émergentes.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Influenza, Human, Severe Acute Respiratory Syndrome.
- epidemiology : Influenza, Human, Severe Acute Respiratory Syndrome.
- pathogenicity : Influenza A Virus, H5N1 Subtype, SARS Virus.
- Communicable Diseases, Emerging, Humans.
Abstract
Two viral agents with RNA genome are responsible for emerging illnesses: influenza virus A/H5N1 and Severe Acute Respiratory Syndrome virus (SARS). For the diagnosis of SARS virus infection, an epidemiological investigation is necessary to know whether the patient has been exposed to a risk in a country where the SARS virus is circulating or whether the patient had worked in a laboratory handling SARS virus. The detection of SARS virus is possible in various clinical samples (including urine) by viral culture or RT-PCR. The handling of those samples and RNA extraction must be performed in a BSL3 laboratory. The SARS virus RT-PCR is poorly sensitive, therefore the test should be performed on samples collected consecutively for several days. In front of a suspicion of A/H5N1, similar procedures are recommended. An epidemiologic investigation is necessary to specify whether the patient stayed in a country where A/H5N1 virus was circulating. Clinical samples needed for a specific diagnosis are: nasopharyngeal, throat-swab or fecal samples, cerebrospinal fluid and blood. The presence of A/H5N1 virus is confirmed by viral isolation or RNA detection by RT-PCR. RNA extraction must be performed in a BSL3 laboratory. For diagnosis of A/H5N1 virus infection, RT-PCR test amplifies specifically a fragment of H5 gene (Hemagglutinin). In french laboratories of medical virology, procedures are ready to diagnose the first case of A/H5N1 virus infection and cases of reemerging SARS virus infection.
PubMed: 16698555
Affiliations:
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pubmed:16698555Le document en format XML
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<term>Influenza, Human (diagnosis)</term>
<term>Influenza, Human (epidemiology)</term>
<term>SARS Virus (pathogenicity)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (epidemiology)</term>
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<term>Maladies transmissibles émergentes</term>
<term>Sous-type H5N1 du virus de la grippe A (pathogénicité)</term>
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<term>Syndrome respiratoire aigu sévère (épidémiologie)</term>
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<term>Syndrome respiratoire aigu sévère</term>
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<front><div type="abstract" xml:lang="en">Two viral agents with RNA genome are responsible for emerging illnesses: influenza virus A/H5N1 and Severe Acute Respiratory Syndrome virus (SARS). For the diagnosis of SARS virus infection, an epidemiological investigation is necessary to know whether the patient has been exposed to a risk in a country where the SARS virus is circulating or whether the patient had worked in a laboratory handling SARS virus. The detection of SARS virus is possible in various clinical samples (including urine) by viral culture or RT-PCR. The handling of those samples and RNA extraction must be performed in a BSL3 laboratory. The SARS virus RT-PCR is poorly sensitive, therefore the test should be performed on samples collected consecutively for several days. In front of a suspicion of A/H5N1, similar procedures are recommended. An epidemiologic investigation is necessary to specify whether the patient stayed in a country where A/H5N1 virus was circulating. Clinical samples needed for a specific diagnosis are: nasopharyngeal, throat-swab or fecal samples, cerebrospinal fluid and blood. The presence of A/H5N1 virus is confirmed by viral isolation or RNA detection by RT-PCR. RNA extraction must be performed in a BSL3 laboratory. For diagnosis of A/H5N1 virus infection, RT-PCR test amplifies specifically a fragment of H5 gene (Hemagglutinin). In french laboratories of medical virology, procedures are ready to diagnose the first case of A/H5N1 virus infection and cases of reemerging SARS virus infection.</div>
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<name sortKey="Hober, D" sort="Hober, D" uniqKey="Hober D" first="D" last="Hober">D. Hober</name>
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<name sortKey="Lobert, P E" sort="Lobert, P E" uniqKey="Lobert P" first="P-E" last="Lobert">P-E Lobert</name>
<name sortKey="Schanen, C" sort="Schanen, C" uniqKey="Schanen C" first="C" last="Schanen">C. Schanen</name>
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<country name="France"><region name="Hauts-de-France"><name sortKey="Goffard, A" sort="Goffard, A" uniqKey="Goffard A" first="A" last="Goffard">A. Goffard</name>
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